|
|
| Sun,Xuerong; Liu,Xinguang; Zhang,Yuehong; Kuang,Xielan; Lv,Bo; Ge,Jian. |
| Mechanical pressure plays an important role in many physiological and pathological processes. Mimicking the mechanical pressure present in vitro is necessary for related research, but usually requires expensive and complicated equipment. In this study we created a simple pressure culture system based on the transwell culture system. By cutting off the top rim of the transwell insert, the cells were compressed between the insert membrane and the well floor. The new pressure culture system was proven effective in that it induced cell morphological change, integrin β1 upregulation, actin polymerization and growth change in rat retinal ganglion cells, human nasopharyngeal carcinoma cells and mice embryonic fibroblasts. Though the pressure value is... |
| Tipo: Journal article |
Palavras-chave: Mechanical pressure; Cell culture; Transwell culture system; Cytoskeleton. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100007 |
| |
